[HTML][HTML] Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis

S Humblet-Baron, B Sather, S Anover… - The Journal of …, 2007 - Am Soc Clin Investig
S Humblet-Baron, B Sather, S Anover, S Becker-Herman, DJ Kasprowicz, S Khim, T Nguyen…
The Journal of clinical investigation, 2007Am Soc Clin Investig
Wiskott-Aldrich syndrome protein (WASp) is essential for optimal T cell activation. Patients
with WAS exhibit both immunodeficiency and a marked susceptibility to systemic
autoimmunity. We investigated whether alterations in Treg function might explain these
paradoxical observations. While WASp-deficient (WASp–/–) mice exhibited normal thymic
Treg generation, the competitive fitness of peripheral Tregs was severely compromised. The
total percentage of forkhead box P3–positive (Foxp3+) Tregs among CD4+ T cells was …
Wiskott-Aldrich syndrome protein (WASp) is essential for optimal T cell activation. Patients with WAS exhibit both immunodeficiency and a marked susceptibility to systemic autoimmunity. We investigated whether alterations in Treg function might explain these paradoxical observations. While WASp-deficient (WASp–/–) mice exhibited normal thymic Treg generation, the competitive fitness of peripheral Tregs was severely compromised. The total percentage of forkhead box P3–positive (Foxp3+) Tregs among CD4+ T cells was reduced, and WASp–/– Tregs were rapidly outcompeted by WASp+ Tregs in vivo. These findings correlated with reduced expression of markers associated with self-antigen–driven peripheral Treg activation and homing to inflamed tissue. Consistent with these findings, WASp–/– Tregs showed a reduced ability to control aberrant T cell activation and autoimmune pathology in Foxp3–/–Scurfy (sf) mice. Finally, WASp+ Tregs exhibited a marked selective advantage in vivo in a WAS patient with a spontaneous revertant mutation, indicating that altered Treg fitness likely explains the autoimmune features in human WAS.
The Journal of Clinical Investigation