[HTML][HTML] A recombinant human hemoglobin with anti-sickling properties greater than fetal hemoglobin
DN Levasseur, TM Ryan, MP Reilly, SL McCune… - Journal of Biological …, 2004 - ASBMB
A new recombinant, human anti-sickling β-globin polypeptide designated β AS3 (βGly 16→
Asp/βGlu 22→ Ala/βThr 87→ Gln) was designed to increase affinity for α-globin. The amino
acid substitutions at β22 and β87 are located at axial and lateral contacts of the sickle
hemoglobin (HbS) polymers and strongly inhibit deoxy-HbS polymerization. The β16
substitution confers the recombinant β-globin subunit (β AS3) with a competitive advantage
over β S for interaction with the α-globin polypeptide. Transgenic mouse lines that …
Asp/βGlu 22→ Ala/βThr 87→ Gln) was designed to increase affinity for α-globin. The amino
acid substitutions at β22 and β87 are located at axial and lateral contacts of the sickle
hemoglobin (HbS) polymers and strongly inhibit deoxy-HbS polymerization. The β16
substitution confers the recombinant β-globin subunit (β AS3) with a competitive advantage
over β S for interaction with the α-globin polypeptide. Transgenic mouse lines that …