Hydrolytic inactivation of a breast carcinoma cell-derived serpin by human stromelysin-3.
To elucidate structure-function relationships of stromelysin-3, a putative matrix
metalloproteinase originally identified at the tumor-stromal cell interface in breast
carcinomas, the human cDNA was expressed in mammalian cells, and its products were
isolated and characterized. In stably transfected cells, stromelysin-3 was recovered as a
complex mixture of species ranging in size from approximately 20 to 65 kDa. Among these
products, a major 45-kDa species with an N terminus of Phe98 and an intact C-terminal …
metalloproteinase originally identified at the tumor-stromal cell interface in breast
carcinomas, the human cDNA was expressed in mammalian cells, and its products were
isolated and characterized. In stably transfected cells, stromelysin-3 was recovered as a
complex mixture of species ranging in size from approximately 20 to 65 kDa. Among these
products, a major 45-kDa species with an N terminus of Phe98 and an intact C-terminal …