Insulin-like growth factor I (IGF-I) is a more potent regulator of gene expression than insulin in primary human myoblasts and myotubes

J Palsgaard, AE Brown, M Jensen, R Borup… - Growth Hormone & IGF …, 2009 - Elsevier
Conventionally, insulin is believed to induce a metabolic response, and IGF-I a mitogenic/
differentiation response in vivo. However, several studies indicate that the roles of insulin
and IGF-I may not be that easy to separate. In this study, insulin and IGF-I specificity in terms
of gene regulation was investigated in primary human skeletal muscle cells before and after
differentiation. Cell cultures were treated with 100nM insulin, IGF-I or nothing for 4h, and
gene expression was subsequently determined using the Affymetrix microarray platform …