Purification of polyphosphoinositides by chromatography on immobilized neomycin.
J Schacht - Journal of Lipid Research, 1978 - ASBMB
The binding of polyphosphoinositides (phosphatidylinositol phosphate and
phosphatidylinositol bisphosphate) to the antibiotic neomycin is utilized for the purification of
these lipids. Neomycin is reductively coupled to reactive glass beads (Glycophase-CPG)
and serves as the stationary phase in column chromatography. A total lipid extract is
prepared from tissues with chloroform-methanol-KC1 or chloroform-methanol-HC1 and
washed once with acidified methanol-water. After the addition of an equal volume of …
phosphatidylinositol bisphosphate) to the antibiotic neomycin is utilized for the purification of
these lipids. Neomycin is reductively coupled to reactive glass beads (Glycophase-CPG)
and serves as the stationary phase in column chromatography. A total lipid extract is
prepared from tissues with chloroform-methanol-KC1 or chloroform-methanol-HC1 and
washed once with acidified methanol-water. After the addition of an equal volume of …