Depletion of TGF-β from fetal bovine serum
T Oida, HL Weiner - Journal of immunological methods, 2010 - Elsevier
TGF-β is one of the key cytokines controlling immune responses. Measuring TGF-β from
culture supernatants in vitro is an important index of immune function. However, fetal bovine
serum (FBS) contains a high level of latent TGF-β that often hampers measuring T cell-
derived TGF-β in culture using FBS-supplemented medium. In this report, we generated anti-
latency associated peptide (LAP) monoclonal antibodies which cross-react with bovine LAP,
and developed a protocol to deplete TGF-β from FBS. This provides the ability to reliably …
culture supernatants in vitro is an important index of immune function. However, fetal bovine
serum (FBS) contains a high level of latent TGF-β that often hampers measuring T cell-
derived TGF-β in culture using FBS-supplemented medium. In this report, we generated anti-
latency associated peptide (LAP) monoclonal antibodies which cross-react with bovine LAP,
and developed a protocol to deplete TGF-β from FBS. This provides the ability to reliably …