Macrophages Are Mediators of Gastritis in Acute Helicobacter pylori Infection in C57BL/6 Mice

M Kaparakis, AK Walduck, JD Price… - Infection and …, 2008 - Am Soc Microbiol
M Kaparakis, AK Walduck, JD Price, JS Pedersen, N Van Rooijen, MJ Pearse, OLC Wijburg…
Infection and immunity, 2008Am Soc Microbiol
Helicobacter pylori is the etiological agent of human chronic gastritis, a condition seen as a
precursor to the development of gastrointestinal ulcers or gastric cancer. This study utilized
the murine model of chronic H. pylori infection to characterize the role of macrophages in the
induction of specific immune responses and gastritis and in the control of the bacterial
burden following H. pylori infection and vaccination. Drug-loaded liposomes were injected
intravenously to deplete macrophages from C57BL/6 mice, and effective removal of CD11b+ …
Abstract
Helicobacter pylori is the etiological agent of human chronic gastritis, a condition seen as a precursor to the development of gastrointestinal ulcers or gastric cancer. This study utilized the murine model of chronic H. pylori infection to characterize the role of macrophages in the induction of specific immune responses and gastritis and in the control of the bacterial burden following H. pylori infection and vaccination. Drug-loaded liposomes were injected intravenously to deplete macrophages from C57BL/6 mice, and effective removal of CD11b+ cells from the spleens and stomachs of mice was confirmed by immunofluorescence microscopy. Transient elimination of macrophages from C57BL/6 mice during the early period of infection reduced the gastric pathology induced by H. pylori SS1 but did not affect the bacterial load in the stomach. These data suggest that macrophages are important to the severity of gastric inflammation during H. pylori infection.
American Society for Microbiology