A novel role of the NRF2 transcription factor in the regulation of arsenite-mediated keratin 16 gene expression in human keratinocytes
H Endo, Y Sugioka, Y Nakagi, Y Saijo… - Environmental health …, 2008 - ehp.niehs.nih.gov
Background Inorganic sodium arsenite (iAs) is a ubiquitous environmental contaminant and
is associated with an increased risk of skin hyperkeratosis and cancer. Objectives We
investigated the molecular mechanisms underlying the regulation of the keratin 16 (K16)
gene by iAs in the human keratinocyte cell line HaCaT. Methods We performed reverse
transcriptase polymerase chain reaction, luciferase assays, Western blots, and
electrophoretic mobility shift assays to determine the transcriptional regulation of the K16 …
is associated with an increased risk of skin hyperkeratosis and cancer. Objectives We
investigated the molecular mechanisms underlying the regulation of the keratin 16 (K16)
gene by iAs in the human keratinocyte cell line HaCaT. Methods We performed reverse
transcriptase polymerase chain reaction, luciferase assays, Western blots, and
electrophoretic mobility shift assays to determine the transcriptional regulation of the K16 …