Defining in vivo dendritic cell functions using CD11c-DTR transgenic mice
L Bar-On, S Jung - Dendritic Cell Protocols, 2010 - Springer
L Bar-On, S Jung
Dendritic Cell Protocols, 2010•SpringerThe study of dendritic cell involvement in complex phenomena that rely on multi-cellular
interactions, such as immune homeostasis, stimulation, and tolerization, called for the
investigation of dendritic cell functions within physiological context. To this end we have
developed a conditional cell ablation strategy that is based on dendritic cell-restricted
expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we
provide basic protocols that describe the use of this prototypic dendritic cell ablation model …
interactions, such as immune homeostasis, stimulation, and tolerization, called for the
investigation of dendritic cell functions within physiological context. To this end we have
developed a conditional cell ablation strategy that is based on dendritic cell-restricted
expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we
provide basic protocols that describe the use of this prototypic dendritic cell ablation model …
Abstract
The study of dendritic cell involvement in complex phenomena that rely on multi-cellular interactions, such as immune homeostasis, stimulation, and tolerization, called for the investigation of dendritic cell functions within physiological context. To this end we have developed a conditional cell ablation strategy that is based on dendritic cell-restricted expression of a Diphtheria Toxin receptor (DTR) using the CD11c/Itgax promoter. Here, we provide basic protocols that describe the use of this prototypic dendritic cell ablation model and highlight pitfalls and strengths of the approach.
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