Chromatographically isolated CD63+CD81+ extracellular vesicles from mesenchymal stromal cells rescue cognitive impairments after TBI

D Kim, H Nishida, SY An, AK Shetty… - Proceedings of the …, 2016 - National Acad Sciences
D Kim, H Nishida, SY An, AK Shetty, TJ Bartosh, DJ Prockop
Proceedings of the National Academy of Sciences, 2016National Acad Sciences
Extracellular vesicles (EVs) secreted by cells present an attractive strategy for developing
new therapies, but progress in the field is limited by several issues: The quality of the EVs
varies with the type and physiological status of the producer cells; protocols used to isolate
the EVs are difficult to scale up; and assays for efficacy are difficult to develop. In the present
report, we have addressed these issues by using human mesenchymal stem/stromal cells
(MSCs) that produce EVs when incubated in a protein-free medium, preselecting the …
Extracellular vesicles (EVs) secreted by cells present an attractive strategy for developing new therapies, but progress in the field is limited by several issues: The quality of the EVs varies with the type and physiological status of the producer cells; protocols used to isolate the EVs are difficult to scale up; and assays for efficacy are difficult to develop. In the present report, we have addressed these issues by using human mesenchymal stem/stromal cells (MSCs) that produce EVs when incubated in a protein-free medium, preselecting the preparations of MSCs with a biomarker for their potency in modulating inflammation, incubating the cells in a chemically defined protein-free medium that provided a stable environment, isolating the EVs with a scalable chromatographic procedure, and developing an in vivo assay for efficacy of the cells in suppressing neuroinflammation after traumatic brain injury (TBI) in mice. In addition, we demonstrate that i.v. infusion of the isolated EVs shortly after induction of TBI rescued pattern separation and spatial learning impairments 1 mo later.
National Acad Sciences