Wortmannin, a specific phosphatidylinositol 3-kinase inhibitor, inhibits adipocytic differentiation of 3T3-L1 cells
K Tomiyama, H Nakata, H Sasa, S Arimura… - Biochemical and …, 1995 - Elsevier
K Tomiyama, H Nakata, H Sasa, S Arimura, E Nishio, Y Watanabe
Biochemical and biophysical research communications, 1995•ElsevierThe effect of wortmannin, which inhibits phosphatidylinositol 3-kinase (PI 3-kinase), on
adipocytic differentiation of 3T3-L1 cells was examined. The extent of differentiation was
evaluated by the staining of adipocytes with Oil Red O and measurement of glycerol 3-
phosphate dehydrogenase (GPDH) activity. Wortmannin, at over 100 nM, significantly
inhibited adipogenesis of cells treated with isobutyl-methylxanthine, dexamethasone, and
insulin with an IC50 value about 50 nM. These results suggest that PI 3-kinase plays a role …
adipocytic differentiation of 3T3-L1 cells was examined. The extent of differentiation was
evaluated by the staining of adipocytes with Oil Red O and measurement of glycerol 3-
phosphate dehydrogenase (GPDH) activity. Wortmannin, at over 100 nM, significantly
inhibited adipogenesis of cells treated with isobutyl-methylxanthine, dexamethasone, and
insulin with an IC50 value about 50 nM. These results suggest that PI 3-kinase plays a role …
The effect of wortmannin, which inhibits phosphatidylinositol 3-kinase (PI 3-kinase), on adipocytic differentiation of 3T3-L1 cells was examined. The extent of differentiation was evaluated by the staining of adipocytes with Oil Red O and measurement of glycerol 3-phosphate dehydrogenase (GPDH) activity. Wortmannin, at over 100 nM, significantly inhibited adipogenesis of cells treated with isobutyl-methylxanthine, dexamethasone, and insulin with an IC50 value about 50 nM. These results suggest that PI 3-kinase plays a role in adipocytic differentiation of 3T3-L1 cells.
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