[HTML][HTML] iCLIP: protein–RNA interactions at nucleotide resolution

I Huppertz, J Attig, A D'Ambrogio, LE Easton, CR Sibley… - Methods, 2014 - Elsevier
Methods, 2014Elsevier
RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene
expression. Precise knowledge about their binding sites is therefore critical to unravel their
molecular function and to understand their role in development and disease. Individual-
nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) identifies protein–
RNA crosslink sites on a genome-wide scale. The high resolution and specificity of this
method are achieved by an intramolecular cDNA circularization step that enables analysis of …
Abstract
RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression. Precise knowledge about their binding sites is therefore critical to unravel their molecular function and to understand their role in development and disease. Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) identifies protein–RNA crosslink sites on a genome-wide scale. The high resolution and specificity of this method are achieved by an intramolecular cDNA circularization step that enables analysis of cDNAs that truncated at the protein–RNA crosslink sites. Here, we describe the improved iCLIP protocol and discuss critical optimization and control experiments that are required when applying the method to new RBPs.
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