Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes).
RM Johnstone, M Adam, JR Hammond, L Orr… - Journal of Biological …, 1987 - ASBMB
Vesicles are released during the in vitro culture of sheep reticulocytes which can be
harvested by centrifugation at 100,000 X g for 90 min. These vesicles contain a number of
activities, characteristic of the reticulocyte plasma membrane, which are known to diminish
or disappear upon reticulocyte maturation. The activities include acetylcholinesterase,
cytochalasin B binding (glucose transporter) nucleoside binding (ie nucleoside transporter),
Na+-independent amino acid transport, and the transferrin receptor. Enzymes of cytosolic …
harvested by centrifugation at 100,000 X g for 90 min. These vesicles contain a number of
activities, characteristic of the reticulocyte plasma membrane, which are known to diminish
or disappear upon reticulocyte maturation. The activities include acetylcholinesterase,
cytochalasin B binding (glucose transporter) nucleoside binding (ie nucleoside transporter),
Na+-independent amino acid transport, and the transferrin receptor. Enzymes of cytosolic …