[HTML][HTML] Methodology for isolation, identification and characterization of microvesicles in peripheral blood
M Jayachandran, VM Miller, JA Heit… - Journal of immunological …, 2012 - Elsevier
RATIONALE: Analyses of circulating cell membrane-derived microvesicles (MV) have come
under scrutiny as potential diagnostic and prognostic biomarkers of disease. However,
methods to isolate, label and quantify MV have been neither systematized nor validated.
OBJECTIVE: To determine how pre-analytical, analytical and post-analytical factors affect
plasma MV counts, markers for cell of origin and expression of procoagulant surface
phosphatidylserine. METHODS AND RESULTS: Peripheral venous blood samples were …
under scrutiny as potential diagnostic and prognostic biomarkers of disease. However,
methods to isolate, label and quantify MV have been neither systematized nor validated.
OBJECTIVE: To determine how pre-analytical, analytical and post-analytical factors affect
plasma MV counts, markers for cell of origin and expression of procoagulant surface
phosphatidylserine. METHODS AND RESULTS: Peripheral venous blood samples were …