Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry
A Clayton, J Court, H Navabi, M Adams… - Journal of …, 2001 - Elsevier
We present a simple yet powerful method for the isolation and analysis of exosomes
released by antigen-presenting cells (APC). Exosomes are small vesicles (40–90 nm)
released by APC, and may have an immuno-regulatory function in vivo. Such exosomes
originate from MHC class II peptide loading compartments and, as such, express high levels
of MHC Class II. We have utilised magnetic beads, coated with monoclonal antibodies
specific for HLA DP, DQ, DR for the specific isolation of exosomes from cell-free …
released by antigen-presenting cells (APC). Exosomes are small vesicles (40–90 nm)
released by APC, and may have an immuno-regulatory function in vivo. Such exosomes
originate from MHC class II peptide loading compartments and, as such, express high levels
of MHC Class II. We have utilised magnetic beads, coated with monoclonal antibodies
specific for HLA DP, DQ, DR for the specific isolation of exosomes from cell-free …