Recruitment of HIV and its receptors to dendritic cell-T cell junctions

D McDonald, L Wu, SM Bohks, VN KewalRamani… - Science, 2003 - science.org
D McDonald, L Wu, SM Bohks, VN KewalRamani, D Unutmaz, TJ Hope
Science, 2003science.org
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity
without themselves becoming infected. Using live-cell microscopy, we found that HIV was
recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T
cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4,
CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs
concentrated HIV to the same region. We propose that contact between dendritic cells and T …
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs concentrated HIV to the same region. We propose that contact between dendritic cells and T cells facilitates transmission of HIV by locally concentrating virus, receptor, and coreceptor during the formation of an infectious synapse.
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