Calcium binding to intestinal goblet cell mucin
JF Forstner, GG Forstner - Biochimica et Biophysica Acta (BBA)-Protein …, 1975 - Elsevier
1. Binding of Ca 2+ to goblet cell mucin of rat small intestine was studied using equilibrium
dialysis against 0.01 M Tris/HCl buffer (pH 7.4) and tracer amounts of 45 CaCl 2. Binding
was found to reach saturation at a Ca free 2+ concentration of 0.1–1.0 mM, to be
independent of temperature (4–37° C), and to increase with increasing pH (5.0–8.7). At low
concentrations of Ca free 2+(< 0.03 mM) the binding curve was sigmoidal, suggesting
positive cooperativity of binding sites and a possible change in the tertiary structure of the …
dialysis against 0.01 M Tris/HCl buffer (pH 7.4) and tracer amounts of 45 CaCl 2. Binding
was found to reach saturation at a Ca free 2+ concentration of 0.1–1.0 mM, to be
independent of temperature (4–37° C), and to increase with increasing pH (5.0–8.7). At low
concentrations of Ca free 2+(< 0.03 mM) the binding curve was sigmoidal, suggesting
positive cooperativity of binding sites and a possible change in the tertiary structure of the …