Detection of HIV-1 in alternative specimen types using the APTIMA® HIV-1 RNA Qualitative Assay
CT Nugent, J Dockter, F Bernardin, R Hecht… - Journal of virological …, 2009 - Elsevier
Peripheral blood mononuclear cells (PBMCs), saliva, seminal plasma, and dried blood spots
were evaluated as specimen types for the APTIMA HIV-1 RNA Qualitative Assay (APTIMA
HIV-1 Assay), which employs a target capture step to recover HIV-1-specific sequences from
complex specimen types. Analytical sensitivity studies were carried out using samples that
were either diluted or eluted with a buffered detergent and spiked with different
concentrations of HIV-1 ranging from 1 to 10,000 copies/mL. PBMC samples spiked with HIV …
were evaluated as specimen types for the APTIMA HIV-1 RNA Qualitative Assay (APTIMA
HIV-1 Assay), which employs a target capture step to recover HIV-1-specific sequences from
complex specimen types. Analytical sensitivity studies were carried out using samples that
were either diluted or eluted with a buffered detergent and spiked with different
concentrations of HIV-1 ranging from 1 to 10,000 copies/mL. PBMC samples spiked with HIV …