[PDF][PDF] NF-κB binds P-TEFb to stimulate transcriptional elongation by RNA polymerase II

M Barboric, RM Nissen, S Kanazawa, N Jabrane-Ferrat… - Molecular cell, 2001 - cell.com
M Barboric, RM Nissen, S Kanazawa, N Jabrane-Ferrat, BM Peterlin
Molecular cell, 2001cell.com
To stimulate transcriptional elongation of HIV-1 genes, the transactivator Tat recruits the
positive transcription elongation factor b (P-TEFb) to the initiating RNA polymerase II
(RNAPII). We found that the activation of transcription by RelA also depends on P-TEFb.
Similar to Tat, RelA activated transcription when tethered to RNA. Moreover, TNF-α triggered
the recruitment of P-TEFb to the NF-κB-regulated IL-8 gene. While the formation of the
transcription preinitiation complex (PIC) remained unaffected, DRB, an inhibitor of P-TEFb …
Abstract
To stimulate transcriptional elongation of HIV-1 genes, the transactivator Tat recruits the positive transcription elongation factor b (P-TEFb) to the initiating RNA polymerase II (RNAPII). We found that the activation of transcription by RelA also depends on P-TEFb. Similar to Tat, RelA activated transcription when tethered to RNA. Moreover, TNF-α triggered the recruitment of P-TEFb to the NF-κB-regulated IL-8 gene. While the formation of the transcription preinitiation complex (PIC) remained unaffected, DRB, an inhibitor of P-TEFb, prevented RNAPII from elongating on the IL-8 gene. Remarkably, DRB inhibition sensitized cells to TNF-α-induced apoptosis. Thus, NF-κB requires P-TEFb to stimulate the elongation of transcription and P-TEFb plays an unexpected role in regulating apoptosis.
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