Antiretroviral-free HIV-1 remission and viral rebound after allogeneic stem cell transplantation: report of 2 cases

TJ Henrich, E Hanhauser, FM Marty… - Annals of internal …, 2014 - acpjournals.org
TJ Henrich, E Hanhauser, FM Marty, MN Sirignano, S Keating, TH Lee, YP Robles, BT Davis…
Annals of internal medicine, 2014acpjournals.org
Background: It is unknown whether the reduction in HIV-1 reservoirs seen after allogeneic
hematopoietic stem cell transplantation (HSCT) with susceptible donor cells is sufficient to
achieve sustained HIV-1 remission. Objective: To characterize HIV-1 reservoirs in blood and
tissues and perform analytic antiretroviral treatment interruptions to determine the potential
for allogeneic HSCT to lead to sustained, antiretroviral-free HIV-1 remission. Design: Case
report with characterization of HIV-1 reservoirs and immunity before and after antiretroviral …
Background
It is unknown whether the reduction in HIV-1 reservoirs seen after allogeneic hematopoietic stem cell transplantation (HSCT) with susceptible donor cells is sufficient to achieve sustained HIV-1 remission.
Objective
To characterize HIV-1 reservoirs in blood and tissues and perform analytic antiretroviral treatment interruptions to determine the potential for allogeneic HSCT to lead to sustained, antiretroviral-free HIV-1 remission.
Design
Case report with characterization of HIV-1 reservoirs and immunity before and after antiretroviral interruption.
Setting
Tertiary care center.
Patients
Two men with HIV with undetectable HIV-1 after allogeneic HSCT for hematologic tumors.
Measurements
Quantification of HIV-1 in various tissues after HSCT and the duration of antiretroviral-free HIV-1 remission after treatment interruption.
Results
No HIV-1 was detected from peripheral blood or rectal mucosa before analytic treatment interruption. Plasma HIV-1 RNA and cell-associated HIV-1 DNA remained undetectable until 12 and 32 weeks after antiretroviral cessation. Both patients experienced rebound viremia within 2 weeks of the most recent negative viral load measurement and developed symptoms consistent with the acute retroviral syndrome. One patient developed new efavirenz resistance after reinitiation of antiretroviral therapy. Reinitiation of active therapy led to viral decay and resolution of symptoms in both patients.
Limitation
The study involved only 2 patients.
Conclusion
Allogeneic HSCT may lead to loss of detectable HIV-1 from blood and gut tissue and variable periods of antiretroviral-free HIV-1 remission, but viral rebound can occur despite a minimum 3-log10 reduction in reservoir size. Long-lived tissue reservoirs may have contributed to viral persistence. The definition of the nature and half-life of such reservoirs is essential to achieve durable antiretroviral-free HIV-1 remission.
Primary Funding Source
Foundation for AIDS Research and National Institute of Allergy and Infectious Diseases.
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