Different role of intracellular loops of glucagon-like peptide-1 receptor in G-protein coupling
A Bavec, M Hällbrink, Ü Langel, M Zorko - Regulatory peptides, 2003 - Elsevier
Previous studies revealed the importance of the third intracellular loop of glucagon-like
peptide-1 receptor (GLP-1R) in coupling to Gs and Gi1 proteins. In order to further study the
signaling mechanisms of GLP-1R, we tested three peptides, corresponding to the
sequences of the first (IC1), the second (IC2), and the third (IC3) intracellular loop of GLP-
1R, for their interactions with heterotrimeric G-proteins of different types (Gαs, Gαo, Gαi1,
and Gα11 plus Gβ1γ2) overexpressed in sf9 cells. IC3 peptide powerfully stimulates all …
peptide-1 receptor (GLP-1R) in coupling to Gs and Gi1 proteins. In order to further study the
signaling mechanisms of GLP-1R, we tested three peptides, corresponding to the
sequences of the first (IC1), the second (IC2), and the third (IC3) intracellular loop of GLP-
1R, for their interactions with heterotrimeric G-proteins of different types (Gαs, Gαo, Gαi1,
and Gα11 plus Gβ1γ2) overexpressed in sf9 cells. IC3 peptide powerfully stimulates all …