Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone

A Adachi, HE Gendelman, S Koenig, T Folks… - Journal of …, 1986 - Am Soc Microbiol
A Adachi, HE Gendelman, S Koenig, T Folks, R Willey, A Rabson, MA Martin
Journal of virology, 1986Am Soc Microbiol
We constructed an infectious molecular clone of acquired immunodeficiency syndrome-
associated retrovirus. Upon transfection, this clone directed the production of infectious virus
particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious
virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines,
indicating the absence of any intracellular obstacle to viral RNA or protein production or
assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely …
We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.
American Society for Microbiology