Effect of macrophage colony-stimulating factor (M-CSF) on mouse immune responses in vivo

T Sakurai, N Wakimoto, M Yamada… - …, 1998 - Taylor & Francis
T Sakurai, N Wakimoto, M Yamada, S Shimamura, K Motoyoshi
Immunopharmacology and immunotoxicology, 1998Taylor & Francis
We examined the effects of recombinant human M-CSF (rhM-CSF) on mouse macrophages
and immune responses in vivo. Intraperitoneal administration of rhM-CSF (20–500 μg/ml)
increased Mac-l+ cell numbers in the peritoneal cavity. The tumoricidal activities of the
macrophages from vehicle-administered (V-MΦ) and from rhM-CSF-administered (M-MΦ)
mice were the same as those observed in vitro. However, when activated by
lipopolysaccharide (LPS), the tumoricidal activity of M-MΦ was stronger than that of V-MΦ …
Abstract
We examined the effects of recombinant human M-CSF (rhM-CSF) on mouse macrophages and immune responses in vivo. Intraperitoneal administration of rhM-CSF (20–500 μg/ml) increased Mac-l+ cell numbers in the peritoneal cavity. The tumoricidal activities of the macrophages from vehicle-administered (V-MΦ) and from rhM-CSF-administered (M-MΦ) mice were the same as those observed in vitro. However, when activated by lipopolysaccharide (LPS), the tumoricidal activity of M-MΦ was stronger than that of V-MΦ. Intravenous administration of rhM-CSF (500 μ g/gk) increased the number of spleen cells. Flow cytometric analysis showed that administration of rhM-CSF increased Mac-1+, B220+ and NK 1.1+ cell counts in the spleen. However, CD4+ and CD8+ cell numbers did not change. Concomitant increases were observed in levels of IL-4 and IL-10 in mouse serum following rhM-CSF administration, but no significant changes were observed in the serum level of IFN-γ.
In experiments involving mouse immune responses, the administration of rhM-CSF reduced the contact sensitivity (CS) reaction against picryl chloride (PC) and augmented IgE production in response to 2,4-dinitrophenyl (DNP), but did not affect the production of either IgM or IgG 1.
These results suggest that administration of rhM-CSF not only activates murine macrophages, but modulates antigen-specific immune responses in vivo.
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