Near-infrared fluorescent proteins for multicolor in vivo imaging
DM Shcherbakova, VV Verkhusha - Nature methods, 2013 - nature.com
Nature methods, 2013•nature.com
Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We
developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and
iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells
and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color
imaging with standard approaches in living cells and mice. The four new iRFPs and the
previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living …
developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and
iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells
and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color
imaging with standard approaches in living cells and mice. The four new iRFPs and the
previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living …
Abstract
Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.
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