[HTML][HTML] Structure-guided engineering enhances a phytochrome-based infrared fluorescent protein

ME Auldridge, KA Satyshur, DM Anstrom… - Journal of Biological …, 2012 - ASBMB
Phytochrome is a multidomain dimeric red light photoreceptor that utilizes a chromophore-
binding domain (CBD), a PHY domain, and an output module to induce cellular changes in
response to light. A promising biotechnology tool emerged when a structure-based
substitution at Asp-207 was shown to be an infrared fluorophore that uses a biologically
available tetrapyrrole chromophore. We report multiple crystal structures of this D207H
variant of the Deinococcus radiodurans CBD, in which His-207 is observed to form a …