Binucleation and polyploidization patterns in developmental and regenerative rat liver growth

P Gerlyng, A Åbyholm, T Grotmol, B Erikstein… - Cell …, 1993 - Wiley Online Library
P Gerlyng, A Åbyholm, T Grotmol, B Erikstein, HS Huitfeldt, T Stokke, PO Seglen
Cell proliferation, 1993Wiley Online Library
The hepatocellular binucleation rate, measured as the percentage of binuclear cells
amongst newly formed bromodeoxyuridine‐labelled and immunostained collage‐nase‐
isolated rat hepatocytes, decreased from 12% to 4% between days 30 and 40 after birth,
rose to 20% between days 50 and 60, and then declined again to the adult rate of about
10% at day 80. During regenerative growth following a two‐thirds partial hepatectomy, the
rate of binucleation declined to about 3%, causing the fraction of binuclear cells to fall from …
Abstract
The hepatocellular binucleation rate, measured as the percentage of binuclear cells amongst newly formed bromodeoxyuridine‐labelled and immunostained collage‐nase‐isolated rat hepatocytes, decreased from 12% to 4% between days 30 and 40 after birth, rose to 20% between days 50 and 60, and then declined again to the adult rate of about 10% at day 80. During regenerative growth following a two‐thirds partial hepatectomy, the rate of binucleation declined to about 3%, causing the fraction of binuclear cells to fall from 27% (before hepactectomy) to 5% (at 45 h after hepactectomy) as pre‐existing binuclear cells replicated and formed mononuclear daughter cells. Essentially all (97%) hepatocytes replicated at least once, starting their DNA synthesis at around 13 h and reaching a peak at 30 h, irrespective of ploidy and nuclearity. At later time points, the diploid hepatocytes had a higher labelling index than the polyploid cells, suggesting a greater tendency to go through several cell cycles.
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