Control of acute, chronic, and constitutive hyperammonemia by wild‐type and genetically engineered Lactobacillus plantarum in rodents
Materials and Methods Bacterial Strains, Plasmids, Media, and Growth Conditions.
Lactobacillus plantarum NCIMB8826 (National Collections of Industrial and Marine Bacteria
Ltd., Aberdeen, UK) and derivatives were grown in Mann, Rogosa, Sharpe (MRS) medium
(Becton Dickinson, Cockeysville, MD) at 37 C without shaking. When appropriate,
chloramphenicol or erythromycin was added to the medium at a final concentration of 10
μg/mL. Strains VL103 (LDH) and VL113 (LDH, NisRK) are derived from NCIMB8826 and are …
Lactobacillus plantarum NCIMB8826 (National Collections of Industrial and Marine Bacteria
Ltd., Aberdeen, UK) and derivatives were grown in Mann, Rogosa, Sharpe (MRS) medium
(Becton Dickinson, Cockeysville, MD) at 37 C without shaking. When appropriate,
chloramphenicol or erythromycin was added to the medium at a final concentration of 10
μg/mL. Strains VL103 (LDH) and VL113 (LDH, NisRK) are derived from NCIMB8826 and are …