High throughput functional analysis of HIV-1 env genes without cloning
JL Kirchherr, X Lu, W Kasongo, V Chalwe… - Journal of virological …, 2007 - Elsevier
Functional human immunodeficiency virus type 1 (HIV-1) env genes have been widely used
for vaccine design, neutralization assays, and pathogenesis studies. However, obtaining
bona fide functional env clones is a time consuming and labor intensive process. A new high
throughput method has been developed to characterize HIV-1 env genes. Multiple rev/env
gene cassettes were obtained from each of seven HIV-1 strains using single genome
amplification (SGA) PCR. The cytomegalovirus (CMV) promoter was amplified separately by …
for vaccine design, neutralization assays, and pathogenesis studies. However, obtaining
bona fide functional env clones is a time consuming and labor intensive process. A new high
throughput method has been developed to characterize HIV-1 env genes. Multiple rev/env
gene cassettes were obtained from each of seven HIV-1 strains using single genome
amplification (SGA) PCR. The cytomegalovirus (CMV) promoter was amplified separately by …