Specificity and detection of insulin-reactive CD4+ T cells in type 1 diabetes in the nonobese diabetic (NOD) mouse

F Crawford, B Stadinski, N Jin… - Proceedings of the …, 2011 - National Acad Sciences
F Crawford, B Stadinski, N Jin, A Michels, M Nakayama, P Pratt, P Marrack, G Eisenbarth…
Proceedings of the National Academy of Sciences, 2011National Acad Sciences
In the nonobese diabetic (NOD) mouse model of type 1 diabetes (T1D), an insulin peptide
(B: 9–23) is a major target for pathogenic CD4+ T cells. However, there is no consensus on
the relative importance of the various positions or “registers” this peptide can take when
bound in the groove of the NOD MHCII molecule, IAg7. This has hindered structural studies
and the tracking of the relevant T cells in vivo with fluorescent peptide-MHCII tetramers.
Using mutated B: 9–23 peptides and methods for trapping the peptide in particular registers …
In the nonobese diabetic (NOD) mouse model of type 1 diabetes (T1D), an insulin peptide (B:9–23) is a major target for pathogenic CD4+ T cells. However, there is no consensus on the relative importance of the various positions or “registers” this peptide can take when bound in the groove of the NOD MHCII molecule, IAg7. This has hindered structural studies and the tracking of the relevant T cells in vivo with fluorescent peptide-MHCII tetramers. Using mutated B:9–23 peptides and methods for trapping the peptide in particular registers, we show that most, if not all, NOD CD4+ T cells react to B:9–23 bound in low-affinity register 3. However, these T cells can be divided into two types depending on whether their response is improved or inhibited by substituting a glycine for the B:21 glutamic acid at the p8 position of the peptide. On the basis of these findings, we constructed a set of fluorescent insulin-IAg7 tetramers that bind to most insulin-specific T-cell clones tested. A mixture of these tetramers detected a high frequency of B:9–23-reactive CD4+ T cells in the pancreases of prediabetic NOD mice. Our data are consistent with the idea that, within the pancreas, unique processing of insulin generates truncated peptides that lack or contain the B:21 glutamic acid. In the thymus, the absence of this type of processing combined with the low affinity of B:9–23 binding to IAg7 in register 3 may explain the escape of insulin-specific CD4+ T cells from the mechanisms that usually eliminate self-reactive T cells.
National Acad Sciences