Recurrence of autoreactive antigen-specific CD4+ T cells in autoimmune diabetes after pancreas transplantation

E Laughlin, G Burke, A Pugliese, B Falk, G Nepom - Clinical immunology, 2008 - Elsevier
CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using
peripheral blood samples after pancreas transplantation in subjects with T1D with clinical
evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the
identification and cloning of antigen-specific autoreactive cells, which were found at several
time points over a multiyear span, in spite of chronic immunosuppression of the subjects.
Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were …