[HTML][HTML] The mRNA cap structure stimulates rate of poly (A) removal and amplifies processivity of degradation
J Martı̂nez, YG Ren, P Nilsson, M Ehrenberg… - Journal of Biological …, 2001 - ASBMB
Poly (A)-specific ribonuclease (PARN) is an oligomeric, processive, and cap-interacting 3′
exonuclease. We have studied how the m 7 G (5′) ppp (5′) G cap structure affects the
activity of PARN. It is shown that the cap has four distinct effects:(i) It stimulates the rate of
deadenylation if providedin cis;(ii) it inhibits deadenylation if provided at high concentration
in trans;(iii) it stimulates deadenylation if provided at low concentration in trans; and (iv) it
increases the processivity of PARN when provided in cis. It is shown that the catalytic and …
exonuclease. We have studied how the m 7 G (5′) ppp (5′) G cap structure affects the
activity of PARN. It is shown that the cap has four distinct effects:(i) It stimulates the rate of
deadenylation if providedin cis;(ii) it inhibits deadenylation if provided at high concentration
in trans;(iii) it stimulates deadenylation if provided at low concentration in trans; and (iv) it
increases the processivity of PARN when provided in cis. It is shown that the catalytic and …