This chapter summarizes the experience at Vernalis over the past decade in developing and
applying fragment-based discovery methods across a range of different targets. The
emphasis will be on the practical aspects of the different biophysical techniques (surface
plasmon resonance (SPR), differential scanning fluorimetry (DSF), isothermal titration
calorimetry, nuclear magnetic resonance, and X-ray crystallography) that can be used to
identify fragments that bind to targets and a discussion of the criteria and strategies for …