Methods for monitoring autophagy using GFP‐LC3 transgenic mice

N Mizushima - Methods in enzymology, 2009 - Elsevier
Several methods are now available for monitoring autophagy. Although biological methods
are useful for cultured cells and homogenous tissues, these methods are not suitable for
determining the autophagic activity of each cell type in heterogenous tissues. Furthermore,
intracellular localization of autophagosomes often provides valuable information. Thus,
morphological assays are still important in many studies. Although electron microscopy has
been the gold standard, recent studies of the molecular mechanism of autophagy have led …