[HTML][HTML] Mucin granule intraluminal organization in living mucous/goblet cells: roles of protein post-translational modifications and secretion
J Perez-Vilar, R Mabolo, CT McVaugh… - Journal of Biological …, 2006 - ASBMB
Recent studies suggest that the mucin granule lumen consists of a matrix meshwork
embedded in a fluid phase. Secretory products can both diffuse, although very slowly,
through the meshwork pores and interact noncovalently with the matrix. Using a green
fluorescent protein-mucin fusion protein (SHGFP-MUC5AC/CK) as a FRAP (fluorescence
recovery after photobleaching) probe, we have assessed in living mucous cells the relative
importance of different protein post-translational modifications on the intragranular …
embedded in a fluid phase. Secretory products can both diffuse, although very slowly,
through the meshwork pores and interact noncovalently with the matrix. Using a green
fluorescent protein-mucin fusion protein (SHGFP-MUC5AC/CK) as a FRAP (fluorescence
recovery after photobleaching) probe, we have assessed in living mucous cells the relative
importance of different protein post-translational modifications on the intragranular …