Production of multiple cytokines by cultured human melanomas
JL Bennicelli, DP Guerry IV - Experimental dermatology, 1993 - Wiley Online Library
JL Bennicelli, DP Guerry IV
Experimental dermatology, 1993•Wiley Online LibraryWe screened a panel of 8 primary and 21 metastatic melanoma cell lines for constitutive
secretion of cytokines. Melanomas expressed bioactivity for TGF‐β (8/25 lines) and IFN
(7/12), but not IL‐2. Immu‐noassays detected IL‐1α (4/25), IL‐1β (12/25), 1L‐6 (13/29), IL‐8
(29/29), TGF‐β2,(5/12) and GM‐CSF (11/29). but not IL‐3, IL‐4, TNF‐α, or IFN‐γ. There was
no preferential association of cytokine production with cells cultured from primary versus
metastatic disease, and only IL‐8 was produced by all lines tested. These data demonstrate …
secretion of cytokines. Melanomas expressed bioactivity for TGF‐β (8/25 lines) and IFN
(7/12), but not IL‐2. Immu‐noassays detected IL‐1α (4/25), IL‐1β (12/25), 1L‐6 (13/29), IL‐8
(29/29), TGF‐β2,(5/12) and GM‐CSF (11/29). but not IL‐3, IL‐4, TNF‐α, or IFN‐γ. There was
no preferential association of cytokine production with cells cultured from primary versus
metastatic disease, and only IL‐8 was produced by all lines tested. These data demonstrate …
Abstract
We screened a panel of 8 primary and 21 metastatic melanoma cell lines for constitutive secretion of cytokines. Melanomas expressed bioactivity for TGF‐β (8/25 lines) and IFN (7/12), but not IL‐2. Immu‐noassays detected IL‐1α (4/25), IL‐1β (12/25), 1L‐6 (13/29), IL‐8 (29/ 29), TGF‐β2, (5/12) and GM‐CSF (11 /29). but not IL‐3, IL‐4, TNF‐α, or IFN‐γ. There was no preferential association of cytokine production with cells cultured from primary versus metastatic disease, and only IL‐8 was produced by all lines tested. These data demonstrate that cultured melanomas produce a variety of cytokines which are potentially capable of influencing tumor growth in vivo.
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