Dystrophinopathy carrier determination and detection of protein deficiencies in muscular dystrophy using lentiviral MyoD-forced myogenesis
ST Cooper, E Kizana, JD Yates, HP Lo, N Yang… - Neuromuscular …, 2007 - Elsevier
The objective of this study is to expand the applications of MyoD-forced myogenesis for
research and diagnosis of human muscle disorders using a lentiviral vector (LVhMyoD) for
efficient trans-differentiation of patient primary cells. LVhMyoD transduced cells readily
formed striated, multinucleate myotubes expressing a wide range of genes associated with
muscular dystrophy (dystrophin, dysferlin, sarcoglycans, caveolin-3) and congenital
myopathy (nebulin, actin, desmin, tropomyosin, troponin). We demonstrate that MyoD gene …
research and diagnosis of human muscle disorders using a lentiviral vector (LVhMyoD) for
efficient trans-differentiation of patient primary cells. LVhMyoD transduced cells readily
formed striated, multinucleate myotubes expressing a wide range of genes associated with
muscular dystrophy (dystrophin, dysferlin, sarcoglycans, caveolin-3) and congenital
myopathy (nebulin, actin, desmin, tropomyosin, troponin). We demonstrate that MyoD gene …