[HTML][HTML] Quantification of newly developed T cells in mice by real-time quantitative PCR of T-cell receptor rearrangement excision circles
AEC Broers, JPP Meijerink, JJM van Dongen… - Experimental …, 2002 - Elsevier
OBJECTIVE: Thymic output of newly developed ab T cells in humans can be measured via
signal joint T-cell receptor rearrangement excision circles (sjTRECs). Deletion of the TCRD
locus via dRec to ψJa recombination during TCRA rearrangement results in the production
of such sjTRECs. The deleting elements dRec and ψJa are highly conserved between
humans and mice and used in a comparable manner. We developed and evaluated a real-
time quantitative PCR (RQ-PCR) to detect and quantify dRec-ψJa sjTRECs in murine …
signal joint T-cell receptor rearrangement excision circles (sjTRECs). Deletion of the TCRD
locus via dRec to ψJa recombination during TCRA rearrangement results in the production
of such sjTRECs. The deleting elements dRec and ψJa are highly conserved between
humans and mice and used in a comparable manner. We developed and evaluated a real-
time quantitative PCR (RQ-PCR) to detect and quantify dRec-ψJa sjTRECs in murine …