Limb and skin abnormalities in mice lacking IKKα

K Takeda, O Takeuchi, T Tsujimura, S Itami, O Adachi… - Science, 1999 - science.org
K Takeda, O Takeuchi, T Tsujimura, S Itami, O Adachi, T Kawai, H Sanjo, K Yoshikawa…
Science, 1999science.org
The gene encoding inhibitor of kappa B (IκB) kinase α (IKKα; also called IKK1) was
disrupted by gene targeting. IKKα-deficient mice died perinatally. In IKKα-deficient fetuses,
limb outgrowth was severely impaired despite unaffected skeletal development. The
epidermal cells in IKKα-deficient fetuses were highly proliferative with dysregulated
epidermal differentiation. In the basal layer, degradation of IκB and nuclear localization of
nuclear factor kappa B (NF-κB) were not observed. Thus, IKKα is essential for NF-κB …
The gene encoding inhibitor of kappa B (IκB) kinase α (IKKα; also called IKK1) was disrupted by gene targeting. IKKα-deficient mice died perinatally. In IKKα-deficient fetuses, limb outgrowth was severely impaired despite unaffected skeletal development. The epidermal cells in IKKα-deficient fetuses were highly proliferative with dysregulated epidermal differentiation. In the basal layer, degradation of IκB and nuclear localization of nuclear factor kappa B (NF-κB) were not observed. Thus, IKKα is essential for NF-κB activation in the limb and skin during embryogenesis. In contrast, there was no impairment of NF-κB activation induced by either interleukin-1 or tumor necrosis factor–α in IKKα-deficient embryonic fibroblasts and thymocytes, indicating that IKKα is not essential for cytokine-induced activation of NF-κB.
AAAS