[HTML][HTML] Nebulin alters cross-bridge cycling kinetics and increases thin filament activation: a novel mechanism for increasing tension and reducing tension cost

M Chandra, R Mamidi, S Ford, C Hidalgo, C Witt… - Journal of biological …, 2009 - ASBMB
Nebulin is a giant filamentous F-actin-binding protein (∼ 800 kDa) that binds along the thin
filament of the skeletal muscle sarcomere. Nebulin is one of the least well understood major
muscle proteins. Although nebulin is usually viewed as a structural protein, here we
investigated whether nebulin plays a role in muscle contraction by using skinned muscle
fiber bundles from a nebulin knock-out (NEB KO) mouse model. We measured force-pCa (−
log [Ca 2+]) and force-ATPase relations, as well as the rate of tension re-development (k tr) …