SV40-transformed simian cells support the replication of early SV40 mutants

Y Gluzman - Cell, 1981 - cell.com
Y Gluzman
Cell, 1981cell.com
CV-1, an established line of simian cells permissive for lytic growth of SV40, were
transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen.
Three transformed lines (COS-1,-3,-7) were established and found to contain T antigen;
retain complete permissiveness for lytic growth of SV40; support the replication of tsA20g
virus at 40 C; and support the replication of pure populations of SV40 mutants with deletions
in the early region. One of the lines (COS-1) contains a single integrated copy of the …
Summary
CV-1, an established line of simian cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1,-3,-7) were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA20g virus at 40 C; and support the replication of pure populations of SV40 mutants with deletions in the early region. One of the lines (COS-1) contains a single integrated copy of the complete early region of SV40 DNA. These cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.
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