A method for identification of HIV gp140 binding memory B cells in human blood
JF Scheid, H Mouquet, N Feldhahn, BD Walker… - Journal of …, 2009 - Elsevier
Antibodies to HIV are potentially important reagents for basic and clinical studies.
Historically, these reagents have been produced by random cloning of heavy and light
chains in phage display libraries [Burton, DR, Barbas, CF III, Persson, MAA, Koenig, S.,
Chanock, RM, and Lerner, RA,(1991), A large array of human monoclonal antibodies to type
1 immnodeficiency virus from combinatorial libraries of asymptomatic seropositive
individuals. Proc. Natl. Acad. Sci. USA 88, 10134–10137.] and electrofusion techniques …
Historically, these reagents have been produced by random cloning of heavy and light
chains in phage display libraries [Burton, DR, Barbas, CF III, Persson, MAA, Koenig, S.,
Chanock, RM, and Lerner, RA,(1991), A large array of human monoclonal antibodies to type
1 immnodeficiency virus from combinatorial libraries of asymptomatic seropositive
individuals. Proc. Natl. Acad. Sci. USA 88, 10134–10137.] and electrofusion techniques …