Immune-mediated mechanisms of parasite tissue sequestration during experimental cerebral malaria

FH Amante, A Haque, AC Stanley… - The Journal of …, 2010 - journals.aai.org
FH Amante, A Haque, AC Stanley, FL Rivera, LM Randall, YA Wilson, G Yeo, C Pieper…
The Journal of Immunology, 2010journals.aai.org
Cerebral malaria is a severe complication of malaria. Sequestration of parasitized RBCs in
brain microvasculature is associated with disease pathogenesis, but our understanding of
this process is incomplete. In this study, we examined parasite tissue sequestration in an
experimental model of cerebral malaria (ECM). We show that a rapid increase in parasite
biomass is strongly associated with the induction of ECM, mediated by IFN-γ and
lymphotoxin α, whereas TNF and IL-10 limit this process. Crucially, we discovered that host …
Abstract
Cerebral malaria is a severe complication of malaria. Sequestration of parasitized RBCs in brain microvasculature is associated with disease pathogenesis, but our understanding of this process is incomplete. In this study, we examined parasite tissue sequestration in an experimental model of cerebral malaria (ECM). We show that a rapid increase in parasite biomass is strongly associated with the induction of ECM, mediated by IFN-γ and lymphotoxin α, whereas TNF and IL-10 limit this process. Crucially, we discovered that host CD4+ and CD8+ T cells promote parasite accumulation in vital organs, including the brain. Modulation of CD4+ T cell responses by helminth coinfection amplified CD4+ T cell-mediated parasite sequestration, whereas vaccination could generate CD4+ T cells that reduced parasite biomass and prevented ECM. These findings provide novel insights into immune-mediated mechanisms of ECM pathogenesis and highlight the potential of T cells to both prevent and promote infectious diseases.
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