Repair of ionizing radiation-induced DNA double-strand breaks by non-homologous end-joining

BL Mahaney, K Meek, SP Lees-Miller - Biochemical Journal, 2009 - portlandpress.com
BL Mahaney, K Meek, SP Lees-Miller
Biochemical Journal, 2009portlandpress.com
DNA DSBs (double-strand breaks) are considered the most cytotoxic type of DNA lesion.
They can be introduced by external sources such as IR (ionizing radiation), by
chemotherapeutic drugs such as topoisomerase poisons and by normal biological
processes such as V (D) J recombination. If left unrepaired, DSBs can cause cell death. If
misrepaired, DSBs may lead to chromosomal translocations and genomic instability. One of
the major pathways for the repair of IR-induced DSBs in mammalian cells is NHEJ (non …
DNA DSBs (double-strand breaks) are considered the most cytotoxic type of DNA lesion. They can be introduced by external sources such as IR (ionizing radiation), by chemotherapeutic drugs such as topoisomerase poisons and by normal biological processes such as V(D)J recombination. If left unrepaired, DSBs can cause cell death. If misrepaired, DSBs may lead to chromosomal translocations and genomic instability. One of the major pathways for the repair of IR-induced DSBs in mammalian cells is NHEJ (non-homologous end-joining). The main proteins required for NHEJ in mammalian cells are the Ku heterodimer (Ku70/80 heterodimer), DNA-PKcs [the catalytic subunit of DNA-PK (DNA-dependent protein kinase)], Artemis, XRCC4 (X-ray-complementing Chinese hamster gene 4), DNA ligase IV and XLF (XRCC4-like factor; also called Cernunnos). Additional proteins, including DNA polymerases μ and λ, PNK (polynucleotide kinase) and WRN (Werner's Syndrome helicase), may also play a role. In the present review, we will discuss our current understanding of the mechanism of NHEJ in mammalian cells and discuss the roles of DNA-PKcs and DNA-PK-mediated phosphorylation in NHEJ.
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