[PDF][PDF] A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major infection

O Filipe-Santos, P Pescher, B Breart, C Lippuner… - Cell host & …, 2009 - cell.com
O Filipe-Santos, P Pescher, B Breart, C Lippuner, T Aebischer, N Glaichenhaus, GF Späth
Cell host & microbe, 2009cell.com
CD4 T helper cells play a central role in the control of infection by intracellular parasites.
How efficiently pathogen-specific CD4 T cells detect infected cells in vivo is unclear. Here,
we employed intravital two-photon imaging to examine the behavior of pathogen-specific
CD4 T cells at the site of Leishmania major infection. While activated CD4 T cells enter the
inflamed tissue irrespective of their antigen specificity, pathogen-specific T cells
preferentially decelerated and accumulated in infected regions of the dermis. Antigen …
Summary
CD4 T helper cells play a central role in the control of infection by intracellular parasites. How efficiently pathogen-specific CD4 T cells detect infected cells in vivo is unclear. Here, we employed intravital two-photon imaging to examine the behavior of pathogen-specific CD4 T cells at the site of Leishmania major infection. While activated CD4 T cells enter the inflamed tissue irrespective of their antigen specificity, pathogen-specific T cells preferentially decelerated and accumulated in infected regions of the dermis. Antigen recognition by CD4 T cells was heterogeneous, involving both stable and dynamic contacts with infected phagocytes. However, not all infected cells induced arrest or deceleration of pathogen-specific T cells, and dense clusters of infected cells were poorly accessible to migrating T cells. Thus, disparities in the dynamics of T cell contacts with infected cells and local variation in T cell access to infected cells are important elements of the host-pathogen interplay.
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