[HTML][HTML] A small region of the natural killer cell receptor, Siglec-7, is responsible for its preferred binding to α2, 8-disialyl and branched α2, 6-sialyl residues: a …
T Yamaji, T Teranishi, MS Alphey, PR Crocker… - Journal of Biological …, 2002 - ASBMB
Siglec-7 is a sialic acid-binding lectin recently identified as an inhibitory receptor on natural
killer cells. Here we characterize the sugar-binding specificity of Siglec-7 expressed on
Chinese hamster ovary cells using polyvalent streptavidin-based glyco-probes. Glyco-
probes carrying unique oligosaccharide structures such as GD3 (NeuAcα2, 8NeuAcα2,
3Galβ1, 4Glc) and LSTb (Galβ1, 3 [NeuAcα2, 6] GlcNAcβ1, 3Galβ1, 4Glc) oligosaccharides
bound to Siglec-7 better than those carrying LSTc (NeuAcα2, 6Galβ1, 4GlcNAcβ1, 3Galβ1 …
killer cells. Here we characterize the sugar-binding specificity of Siglec-7 expressed on
Chinese hamster ovary cells using polyvalent streptavidin-based glyco-probes. Glyco-
probes carrying unique oligosaccharide structures such as GD3 (NeuAcα2, 8NeuAcα2,
3Galβ1, 4Glc) and LSTb (Galβ1, 3 [NeuAcα2, 6] GlcNAcβ1, 3Galβ1, 4Glc) oligosaccharides
bound to Siglec-7 better than those carrying LSTc (NeuAcα2, 6Galβ1, 4GlcNAcβ1, 3Galβ1 …