Huntingtin-associated protein (HAP1): discrete neuronal localizations in the brain resemble those of neuronal nitric oxide synthase.

XJ Li, AH Sharp, SH Li, TM Dawson… - Proceedings of the …, 1996 - National Acad Sciences
XJ Li, AH Sharp, SH Li, TM Dawson, SH Snyder, CA Ross
Proceedings of the National Academy of Sciences, 1996National Acad Sciences
Huntington disease stems from a mutation of the protein huntingtin and is characterized by
selective loss of discrete neuronal populations in the brain. Despite a massive loss of
neurons in the corpus striatum, NO-generating neurons are intact. We recently identified a
brain-specific protein that associates with huntingtin and is designated huntingtin-associated
protein (HAP1). We now describe selective neuronal localizations of HAP1. In situ
hybridization studies reveal a resemblance of HAP1 and neuronal nitric oxide synthase …
Huntington disease stems from a mutation of the protein huntingtin and is characterized by selective loss of discrete neuronal populations in the brain. Despite a massive loss of neurons in the corpus striatum, NO-generating neurons are intact. We recently identified a brain-specific protein that associates with huntingtin and is designated huntingtin-associated protein (HAP1). We now describe selective neuronal localizations of HAP1. In situ hybridization studies reveal a resemblance of HAP1 and neuronal nitric oxide synthase (nNOS) mRNA localizations with dramatic enrichment of both in the pedunculopontine nuclei, the accessory olfactory bulb, and the supraoptic nucleus of the hypothalamus. Both nNOS and HAP1 are enriched in subcellular fractions containing synaptic vesicles. Immunocytochemical studies indicate colocalizations of HAP1 and nNOS in some neurons. The possible relationship of HAP1 and nNOS in the brain is reminiscent of the relationship of dystrophin and nNOS in skeletal muscle and suggests a role of NO in Huntington disease, analogous to its postulated role in Duchenne muscular dystrophy.
National Acad Sciences