An estimation of the number of malaria sporozoites ejected by a feeding mosquito

R Rosenberg, RA Wirtz, I Schneider, R Burge - Transactions of the Royal …, 1990 - Elsevier
Restrained Anopheles stephensi mosquitoes infected with Plasmodium falciparum were
made to produce time-dependent series of saliva droplets in mineral oil. The relative volume
of each droplet and the number of sporozoites each contained were determined
microscopically; gland sporozoites were estimated with an enzyme-linked immunosorbent
assay. Median gland infection was 8170 and median number of sporozoites ejected was 15
(range, 0–978). Inoculumsize was positively correlated to the number of sporozoites in the …