Detection and discrimination of individual viruses by flow cytometry.

M Hercher, W Mueller… - Journal of Histochemistry …, 1979 - journals.sagepub.com
M Hercher, W Mueller, HM Shapiro
Journal of Histochemistry & Cytochemistry, 1979journals.sagepub.com
A new flow cytometer with a very small observation volume has been developed to detect
individual viruses with good resolution, and has been used to discriminate between two
types of viral particles based on differences in their light scattering. Measurements of light
scattering and fluorescence made with such an instrument can provide a basis for
quantitative analysis and sorting of viruses and other particles in the micron and submicron
size range.
A new flow cytometer with a very small observation volume has been developed to detect individual viruses with good resolution, and has been used to discriminate between two types of viral particles based on differences in their light scattering. Measurements of light scattering and fluorescence made with such an instrument can provide a basis for quantitative analysis and sorting of viruses and other particles in the micron and submicron size range.
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