Chondrocyte apoptosis induced by collagen degradation: inhibition by caspase inhibitors and IGF-1
MY Lo, HT Kim - Journal of orthopaedic research, 2004 - Elsevier
The main objective of this study was to test the effectiveness of candidate apoptosis
inhibitors in limiting chondrocyte apoptosis induced by collagen degradation. Primary
human chondrocytes were isolated from normal articular cartilage and grown in monolayer
culture. Collagenase was added to the cells in the presence and absence of caspase
inhibitors and insulin like growth factor (IGF)-1. The amount of chondrocyte apoptosis was
measured using an enzyme linked immunosorbent assay for nucleosomes, a specific and …
inhibitors in limiting chondrocyte apoptosis induced by collagen degradation. Primary
human chondrocytes were isolated from normal articular cartilage and grown in monolayer
culture. Collagenase was added to the cells in the presence and absence of caspase
inhibitors and insulin like growth factor (IGF)-1. The amount of chondrocyte apoptosis was
measured using an enzyme linked immunosorbent assay for nucleosomes, a specific and …