[HTML][HTML] Proinsulin disulfide maturation and misfolding in the endoplasmic reticulum
Upon nonreducing Tris-Tricine-urea-SDS-PAGE, newly synthesized proinsulin from
pancreatic islets of normal rodents forms a band fast mobility representing the native
disulfide isomer, which is efficiently secreted. In addition at least two slower migrating"
isomer 1 and 2" bands are recovered, not discernible under reducing conditions, which
represent minor species that exhibit less efficient secretion. Although rats and mice have two
proinsulin genes, three distinct migrating species are also produced upon proinsulin …
pancreatic islets of normal rodents forms a band fast mobility representing the native
disulfide isomer, which is efficiently secreted. In addition at least two slower migrating"
isomer 1 and 2" bands are recovered, not discernible under reducing conditions, which
represent minor species that exhibit less efficient secretion. Although rats and mice have two
proinsulin genes, three distinct migrating species are also produced upon proinsulin …